Supplementary Figure 8: Statistical testing for binding overlap between BLIMP1, Ap2γ and PRDM14 and co-expression of BLIMP1, AP2γ and PRDM14 induces PGC-like cell fate in vitro.
From: A tripartite transcription factor network regulates primordial germ cell specification in mice
(a). Number of observed and expected overlap in genomic binding sites of BLIMP1, AP2γ and PRDM14, and a scatterplot showing the observed against expected overlap in genomic binding sites of: 1. AP2γ and BLIMP1, 2. BLIMP1 and PRDM14, and 3. AP2γ and PRDM14. The P-value for the enrichment of overlap in binding sites is P<0.0001 for all comparisons. (b) A contingency table for the calculation of a chi-square p-value for the overlap of genes bound by different combinations of BLIMP1, AP2γ and PRDM14. The calculated p-value based on this table was p<1×10−299. The total number of genes (34274) represents the number of unique gene identifiers in Ensembl that were a basis for the gene annotation of the respective transcription factor binding sites. (c). RT–qPCR analysis of sorted fluorescent PGCLCs on Day2 and 4 of either cytokine or doxycycline induction, as well as EpiLCs. The experiment is the second of two experiments performed. The first experiment is shown in Fig. 8.
