Supplementary Figure 2: Comparable amounts of Kip3 and Kip3ΔT-LZ on track microtubules, related to the sliding assay in Fig. 1b.
From: Microtubule-sliding activity of a kinesin-8 promotes spindle assembly and spindle-length control

(LEFT) TMR-labeled Kip3 or Kip3ΔT-LZ decorated track microtubules in the sliding assay. Scale bar: 5 μm. (RIGHT) Quantification of fluorescence intensity of Kip3 and Kip3ΔT-LZ along track microtubules at the beginning of sliding assays. Shown is mean±s.e.m. (N = 30 microtubules). Protein input: 150 nM.