Supplementary Figure 3: The spindle is trapped in a static actin network if the function of Rab11a-positive vesicles is blocked.
From: Vesicles modulate an actin network for asymmetric spindle positioning
(a) Image correlation analysis of actin network dynamics in live oocytes expressing EGFP-UtrCH to label F-actin together with mCherry-Rab11a (Control) or mCherry-Rab11a S25N (Rab11a S25N). Each image in a time series (time interval 1.57 s) was correlated with the first image. For details see Experimental Procedures. The number of analysed oocytes is specified in italics (aggregation over 2 independent experiments). Data are mean, with error bars displaying s.d. (b) Live oocytes expressing EGFP-UtrCH to label F-actin together with mCherry-Rab11a (Control) or mCherry-Rab11a S25N (Rab11a S25N). Projections are time-coloured in RGB. Scale bar, 5 μm.
