Supplementary Figure 4: Network stabilization with jasplakinolide prevents asymmetric spindle positioning.

(a) Spindle movements (magenta, EGFP-MAP4; microtubules, merged with differential interference contrast [DIC]) in live oocytes treated with DMSO (Control) or oocytes treated with 50 nM jasplakinolide (Jasplakinolide). White ovals mark initial spindle positions. Scale bar, 10 μm. (b) The efficiency of asymmetric spindle positioning in control and jasplakinolide treated oocytes is shown. The number of analysed oocytes is specified in italics (aggregation over 2-5 independent experiments). (c) The spindle was tracked in oocytes in 3D data sets (11 sections, every 8 μm) as shown in (a) and spindle movements were plotted. The number of analysed oocytes is specified in italics (aggregation over 2-5 independent experiments). Data are mean, with error bars displaying s.d. (d) The spindle speeds were determined from the plots in (c). The number of analysed oocytes is specified in italics (aggregation over 2-5 independent experiments). Box plot as in Fig. 1e.