Supplementary Figure 7: Localization of the network’s actin nucleators in the presence and absence of Rab11-a positive vesicles.
From: Vesicles modulate an actin network for asymmetric spindle positioning
(a) Two additional examples of mEGFP-Spire2 localization in anti-GFP immunogold labelled oocytes. Boxed regions are magnified next to overview. Representative examples from 5 independent experiments (23 oocytes total). Scale bar, 200 nm. (b) Z-projection (5 sections, every 0.66 μm) of live oocytes expressing mEGFP-Spire1 with either mCherry-Rab11a (top panel) or mCherry-Rab11a S25N (bottom panel). Boxed regions are magnified in inset. Representative examples from 2 independent experiments (>15 oocytes total for each condition). Scale bar, 10 μm. (c) Z-projection (5 sections, every 0.66 μm) of live oocytes expressing Fmn2-mCherry together with mEGFP-Spire1 and mEGFP-Rab11a (top panel) or mEGFP-Spire1 and mEGFP-Rab11a S25N (bottom panel). Representative examples from 3 independent experiments (>30 oocytes total for each condition). Scale bar, 10 μm.
