Supplementary Figure 8: BFA addition releases the network’s actin nucleators from vesicles.
From: Vesicles modulate an actin network for asymmetric spindle positioning
(a) Control for BFA addition experiment in Fig. 5i. mEGFP-Spire2 localization upon addition of methanol. (z-projection, 5 sections, every 0.66 μm). Scale bar, 10 μm. Boxed regions are magnified below. (b) mEGFP-Spire1 localization in live oocytes upon addition of 10 μM BFA (z-projection, 5sections, every 0.66 μm). Representative example from 4 independent experiments (17 oocytes total). Scale bar, 10 μm. Boxed region is magnified below. (c) Fmn2-mCherry localization in live oocytes co-overexpressing mEGFP-Spire1 upon addition of 10 μM BFA. Only a single section is shown. Representative example from 2 independent experiments (6 oocytes total). Scale bar, 10 μm. Boxed region is magnified below.
