Supplementary Figure 1: Loss of Rab11a-positive vesicles leads to mislocalization of the vesicles’ cargo transferrin.
From: Vesicles modulate an actin network for asymmetric spindle positioning
(a) Transferrin localization in oocytes expressing mEGFP-Rab11a and oocytes expressing dominant-negative Rab11a (Rab11a S25N) (z-projection, 3 sections, every 0.66 μm). Boxed regions are magnified in inset. Scale bar, 10 μm. (b) The number of transferrin-positive vesicles in control and dominant-negative Rab11a expressing oocytes is shown. Vesicles were identified and counted with the spot detection function of Imaris in an oocyte segment of 20×20×20 μm3. The number of analysed oocytes is specified in italics (aggregation over 2 independent experiments). Data are mean, with error bars displaying s.d. P values were calculated with Student’s t-test.
