Supplementary Figure 3: Riquiqui and Minibrain regulate SWH pathway activity.

Wing imaginal discs from third instar D. melanogaster larvae harbouring the following elements: (a-a”) en-Gal4, UAS-EYFP and th-lacZ; (b-b”) en-Gal4, UAS-riq and th-lacZ; (c-c”) en-Gal4, UAS-mnb and th-lacZ; (d,d’) hh-Gal4, UAS-EYFP and ex-lacZ; (e,e’) hh-Gal4, UAS-riq and ex-lacZ; (f,f’) hh-Gal4, UAS-mnb and ex-lacZ; (g,g’) hh-Gal4, UAS-riq, UAS-mnb and ex-lacZ. Yki activity (grey) was reported by th-lacZ (a–c) or ex-lacZ (d–g). All transgenes were expressed in the posterior compartment of wing imaginal discs; Ci expression (red) marks the anterior compartment. Merged images are shown in (a”–c” and in d’–g’). (h) An adult female fly expressing both riq and mnb under control of the rn-Gal4 driver. Unlike driving expression of riq and mnb alone, driving expression of both transgenes together caused severe folding and crumpling of wings, which prevented quantification of their size. (e) Quantification of LacZ expression driven by ex-lacZ, ban-lacZ or th-lacZ in wing imaginal discs displayed in Fig. 3 and Supplementary Fig. S3a–c. Anterior is to the left. For each disc fluorescence intensity was measured by ImageJ in two boxes of defined size in both the anterior (ant.) and posterior (post.) compartments. These values were averaged and plotted for each experiment. Scale bar represents 50 μm.