Supplementary Figure 1: Dachsous promotes localization of Riquiqui to apical junctions and cell membranes.
From: Riquiqui and Minibrain are regulators of the Hippo pathway downstream of Dachsous
(a-c”) X-Z cross-sections of D. melanogaster third instar larval wing imaginal discs expressing the following elements: (a, a’, a”) rn-Gal4, UAS-riq; (b, b’, b”) rn-Gal4, UAS-riq and UAS-ds; (c, c’, c”) rn-Gal4, UAS-riq and UAS-dsRi. Riq is in white, E-cadherin marks the adherens junction in green. Arrows indicate Riq protein that is present at apical junctions in Ds-expressing tissues. Magnified images are inset in (a) (a’) (b) and (b’). Merged images are on the right. Riq was junctionally localised immediately apical to E cadherin. Riq was largely absent from apical junctions of tissues where Ds was depleted by RNAi. (d and e) D. melanogaster S2 cells expressing either Riq (d) or Riq and DsΔECD (e). Riq is in red, DsΔECD is green, Direct interference contrast (DIC) images are grey. Arrows indicate Riq protein that is present at cell membranes in DsΔECD-expressing cells. Scale bar represents 10 μm in (a-c”) and 5 μm in (d) and (e).
