Supplementary Figure 2: UAS-riq RNAi transgenes decrease Riq expression.
From: Riquiqui and Minibrain are regulators of the Hippo pathway downstream of Dachsous
(a) A wing imaginal disc from a third instar D. melanogaster larva expressing UAS-riqi1 under the control of hh-Gal4. Riq expression (grey in a) was assessed using an antibody directed against DCAF7 (human homologue of Riq). Cubitus interruptus (Ci) expression (red in a’) marked the anterior compartment of wing imaginal discs. A merged image is shown in (a”). (b) The level of riq mRNA relative to the control actin5C mRNA, assessed by QRT-PCR, in wing imaginal discs harbouring the following transgenes: hh-Gal4 and UAS-EYFP; or hh-Gal4 and UAS-riqi1. Data is presented as mean±SEM,n = 3, ** = p<0.01. Scale bar represents 50 μm in (a). Statistics source data for Supplementary Fig. S2b is available in Supplementary Table S1.
