Supplementary Figure 2: Q-bodies associate with specific sub-cellular structures. | Nature Cell Biology

Supplementary Figure 2: Q-bodies associate with specific sub-cellular structures.

From: Spatial sequestration of misfolded proteins by a dynamic chaperone pathway enhances cellular fitness during stress

Supplementary Figure 2

(a) Two-color images of Z-focal plans (0.2 μm intervals; obtained after 10 min at 37 °C) of cells expressing Ubc9ts–CHFP (red) or Ubc9ts–GFP (green) and the following markers: GFP–Snc1 (early endosomes; green), GFP–Pep12 (late endosomes; green), CHFP–Atg8 (autophagic vesicles; red); the vacuole was imaged by treating cells with MDY64 (blue). Scale bars equal 1 μm. (b) Cells expressing Spc42–GFP (green) and Ubc9ts–CHFP (red) were imaged 15 min after a shift from 28 °C to 37 °C. Scale bars equal 1 μm.

Back to article page