Supplementary Figure 3: Hsp70–Hsp90 chaperones promote the maturation and degradation of Q-bodies. | Nature Cell Biology

Supplementary Figure 3: Hsp70–Hsp90 chaperones promote the maturation and degradation of Q-bodies.

From: Spatial sequestration of misfolded proteins by a dynamic chaperone pathway enhances cellular fitness during stress

Supplementary Figure 3

(a) Schematic of the Hsp70 chaperone system and its connection to the Hsp90 system. (b) Ubc9ts–GFP was expressed in WT and ssa1-4 ssa2Δssa3Δssa4Δ (herein ssa1ts ssa2Δssa3Δssa4Δ) background at 28 °C in galactose medium and shifted at 37 °C in glucose medium. 5 min series of images shows Ubc9ts–GFP in these strains over 30 min. Scale bars equal 1 μm. (c) Representation of the average number of puncta per cell in the WT (purple squares) and the ydj1Δ (orange circles) strains over time. Puncta assessed from a total population of n = 38 cells over three independent experiments (1 field counted per experiment). (*) p<0.05 (**) p<0.005 compared to WT for the same indicated time. (d) Ubc9ts–GFP expressed in WT, hlj1Δ and hlj1Δydj1-151 cells was imaged as in b. Scale bar equals 1 μm. (e) Average number of puncta per cell in the WT (purple squares) and the sse1Δ (green triangles) strains over timePuncta assessed from a total population of n = 16 cells over three independent experiments (1 field counted per experiment). (**) p<0.005 compared to the WT for the same indicated time.

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