Supplementary Figure 5: Different types of misfolded proteins, but not amyloids, co-localize to and are processed together via the same Q-bodies.

(a) WT cells expressing Ubc9ts–CHFP and Luc-GFP were grown at 28 °C in galactose medium and imaged at 37 °C in glucose medium. 5 min series of images show Ubc9ts–CHFP in red and Luc-GFP in green. Cells expressing Ubc9-GFP were similarly prepared and imaged 15 min after the shift. Scale bar equals 1 μm. (b) WT cells expressing CHFP-VHL were grown as in a. 5 min series of images shows CHFP-VHL over 30 min. Scale bar equals 1 μm. (c) WT cells expressing GFP-VHL (green) and Rnq1-CHFP (red) were grown and imaged as in a. Scale bar equals 1 μm. (d) Cells expressing Hsp42–GFP and Htt-Q97-CHFP were grown at 28 °C in galactose medium and shifted for 10 min at 37 °C in glucose medium. Two-color images (deconvolved in the lower panel) show Hsp42–GFP signal in green and Htt-Q97-CHFP signal in red. Scale bar equals 1 μm. (e) Cells expressing Hsp104–GFP (green) and Htt-Q97-CHFP (red) were grown and imaged as in d. Scale bars equal 1 μm.