Supplementary Figure 5: Four additional examples for securin-mEGFP degradation in correlation with unaligned chromosomes induced by low dose nocodazole.
From: Kinetic framework of spindle assembly checkpoint signalling
HeLa cells expressing H2B-mCherry and securin-mEGFP were imaged and analyzed as in Fig. 5f,g. Dark red indicates time intervals with >5 unaligned chromosomes, light red indicates time intervals with 2–5 unaligned chromosomes, orange indicates one unaligned chromosome, green indicates alignment of all chromosomes. Solid lines indicate pre-anaphase stages, dashed lines indicate anaphase stages. (a–d) Background-subtracted and bleach corrected total fluorescence normalized to prometaphase. (a) Cell treated with 6 ng/ml nocodazole. (b) 25 ng/ml nocodazole. (c, d) 12 ng/ml nocodazole. (e-h) Background-subtracted raw measurements of the cells shown in a-d.
