Supplementary Figure 4: Size-exclusion chromatography of RPE1 cells treated with Taxol and nocodazole.
From: The spindle assembly checkpoint works like a rheostat rather than a toggle switch
Extracts from (a) Taxol- and (b) nocodazole-treated RPE1 cells were fractionated by size-exclusion chromatography and analysed by quantitative immunoblotting with the indicated antibodies. Signal intensities (right panels) are relative to the highest value for each protein. Representative of three independent experiments. Molecular mass markers on the left. (c) Absolute amount of checkpoint proteins per Cdc20 in the MCC fractions of both Taxol- and nocodazole-treated RPE1 cells (n = 3). Taxol values were set to 1. Error bars indicate s.e.m. and are representative of three experiments.
