Supplementary Figure 6: Hh protein levels are not affected by the manipulation of cytoneme length. | Nature Cell Biology

Supplementary Figure 6: Hh protein levels are not affected by the manipulation of cytoneme length.

From: Cytonemes are required for the establishment of a normal Hedgehog morphogen gradient in Drosophila epithelia

Supplementary Figure 6

Either the expression of CPa-RNAi (a), Scar-RNAi (b), Pico-RNAi (c), UAS.Flo2 (d) or Flo2-RNAi (e) in the dorsal compartment of wing discs using ap.Gal4 affects Hh protein levels. Hh levels are similar in the mutant (dorsal) and the control (ventral) parts of the P compartment after 24 hours of expression. GFP is co-expressed to label the dorsal compartment, and α-Hh labels the P compartment. A hatched line indicates the D/V border. n∼8 discs per treatment. Anterior is to the left.

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