Supplementary Figure 7: Interfering with exovesicle production/release affects the length of the Hh activity gradient. | Nature Cell Biology

Supplementary Figure 7: Interfering with exovesicle production/release affects the length of the Hh activity gradient.

From: Cytonemes are required for the establishment of a normal Hedgehog morphogen gradient in Drosophila epithelia

Supplementary Figure 7

(a–d) Fluorescence images of wing discs, in which the Hh activity gradient in A compartment cells is visualised using the Ptc-promotor-trap::GFP reporter. A magnification of the Ptc signal is shown at the bottom. RNAi-constructs were expressed in the P compartment using hh.Gal4. (a) Wild-type control. (b) Alix-RNAi. (c) TSG101-RNAi. (d) HRS-RNAi. In the RNAi wing discs (b-d), the gradients are shorter than in the control (a). In all images, anterior is to the left. (e) Box plot comparing gradient length between control discs and the RNAi treatments. There were no significant differences among the five RNAi treatments, but a significant difference between the control and each of the six RNAi treatments. See Methods for details on the statistical analysis.

Back to article page