Supplementary Figure 2: Mitochondrial morphology and MCU expression in MCU−/− mice. | Nature Cell Biology

Supplementary Figure 2: Mitochondrial morphology and MCU expression in MCU−/− mice.

From: The physiological role of mitochondrial calcium revealed by mice lacking the mitochondrial calcium uniporter

Supplementary Figure 2

(a) Electron micrographic images of both fetal (E12.5) and adult liver and heart. WT and MCU−/− tissues show similar mitochondrial morphology and abundance. Scale bars = 500 nm. (b) Analysis of MCU expression in tissues from WT and MCU−/− mice. Protein lysates were identical to those shown in Figure 1d, however a different antibody against MCU (Sigma) recognizing the N-terminal region of MCU was used. Tubulin, identical to Figure 1d, is again used as a loading control. (c) Protein expression in MEFs obtained from wild type or MCU−/− embryos. GAPDH is used as a loading control.

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