Supplementary Figure 4: Basal oxygen consumption and authophagic flux in MCU−/− cells and tissues. | Nature Cell Biology

Supplementary Figure 4: Basal oxygen consumption and authophagic flux in MCU−/− cells and tissues.

From: The physiological role of mitochondrial calcium revealed by mice lacking the mitochondrial calcium uniporter

Supplementary Figure 4

(a) Relative oxygen consumption measured using a Seahorse X-24 analyzer analyzing WT and MCU−/− MEFs under basal conditions with glucose as the substrate (25 mM), or a similar analysis using pyruvate (2 mM) or in the presence of galactose (25 mM). Results are the average +/− S.E.M. of three independent experiments each performed in quadruplicate. b) Autophagic flux as determined by levels of p62 and the ratio of LC3I/LC3II. MEF cells were shifted to a low nutrient media (Hanks buffered salt solution; t = 0) and subsequently assessed for markers of autophagic flux. Consistent with increased autophagic flux, starvation led to decreased levels of p62 and an increased LC3II/LC3I ratio. However, this response was similar between WT and in MCU−/− MEFs. A representative example from three similar experiments is shown. (c) Similar assessment in vivo in both liver and heart tissues under randomly fed conditions. Again, no consistent differences were observed between genotypes. GAPDH is shown as a loading control.

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