Supplementary Figure 1: Additional genetic analyses of SelR and Semaphorin/Plexin/Mical signalling.
From: SelR reverses Mical-mediated oxidation of actin to regulate F-actin dynamics
a–b, Elevating the levels of SelR in a wild-type background generates abnormally bent bristles (a) that resemble Mical loss-of-function homozygous (Mical−/−) mutant and Mical dominant-negative mutant bristles (b; 1). c, Bristle specific expression of high levels of SelR in a Mical bristle overexpression background generates bristles that resemble Mical−/− mutant bristles (b). d, The bristle defects that result from 1 copy overexpression of SelR in bristles is strongly enhanced by loss of 1 copy of Mical (Mical heterozygous [Mical+/−] mutants). MicalDf(3R)swp2 allele. Chi-Square Test; ***P<0.0001. n = 20 animals per genotype. Replicated in at least 2 independent experiments (separate crosses) per genotype. e-g, SelR counteracts Semaphorin/Plexin A (PlexA) signaling. e, Overexpression of PlexA in bristles (x2 Bristle PlexA) results in bristle branching 1. Overexpression of SelR in bristles strongly suppresses these PlexA-dependent defects (x2 Bristle PlexA + x1 Bristle SelR). t-test; ***P<0.0001. n = 20 animals per genotype. Mean + standard error of the mean (SEM). Replicated in at least 2 independent experiments (separate crosses) per genotype. f, A loss-of-function/dominant negative mutant of PlexA (PlexA without its cytoplasmic region [PlexAΔCyto]; 1,2,3,4) generates bristles defects when it is expressed in bristles (x1 Bristle PlexAΔCyto; 1). Bristle overexpression of 1 copy of SelR strongly enhances these PlexAΔCyto bristle defects (x1 Bristle PlexAΔCyto+x1 Bristle SelR). In contrast, the catalytically dead SelRC124S does not enhance these PlexAΔCyto bristle defects (x1 Bristle PlexAΔCyto+x1 Bristle SelRC124S)but suppresses them. Chi-Square Test; ***P<0.0001. n = 8 animals per genotype. Replicated in at least 2 independent experiments (separate crosses) per genotype. g, A SelR heterozygous loss-of-function mutant (SelRDelta3 mutant/+) enhances PlexA-dependent bristle defects. n = 20 animals per genotype. Mean ± standard error of the mean (SEM). Replicated in at least 2 independent experiments (separate crosses) per genotype.
