Supplementary Figure 5: In situ detection of AKT phosphorylation in oocytes stimulated with AREG when in complex with cumulus cells.
From: Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
A. CEOs were cultured with or without AREG for 150 min; at the end of the incubation, oocytes were freed of cumulus cells and stained for phospho-AKT (green). B. Quantification of the intensity of the phospho-AKT from different experiments (mean ± SEM; n = 3). *p<0.05 vs CEO. The scale bar corresponds to 10 μm.
