Supplementary Figure 6: mTOR inhibitors block the reporter translation stimulated by AREG.
From: Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
A. Representative Western blot of phosphorylation of ribosomal protein S6 (rpS6) in oocytes cultured as CEO in medium containing 50 nM of rapamycin (mTOR inhibitor) or 100nM of INK128 (selective TORC1/2 inhibitor) for 150min. Tubulin was used as loading control. B. Quantification of the intensity of the phospho-rpS6 immunoreactive band from different experiments (mean ± SEM; n = 3) *P<0.05 vs AREG.
