Supplementary Figure 4: Phosphorylation of AKT (Ser 473) in oocytes is dependent on the presence of somatic cells.
From: Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
A. Representative time course of AKT Ser 473 phosphorylation in oocytes cultured as cumulus enclosed oocytes (CEOs). CEOs were incubated in medium with AREG and 2 μM milrinone to prevent maturation. At each time oocytes were freed of surrounding cumulus and used for Western blot analysis. Total AKT was used as loading control. A representative experiment of the three performed is reported. B. Representative Western blots of phosphorylated AKT in oocytes cultured as CEO or denuded as indicated times with or without AREG. Total AKT was used as loading control.
