Supplementary Figure 1: AREG-dependent stimulation of reporter translation is absent when meiotic reentry is prevented.
From: Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
A. Cumulus enclosed oocytes were injected with TPX2 3’UTR Renilla luciferase reporter and cultured overnight in medium that enables maturation to MII or in medium that prevents maturation (2 μM milrinone) with or without AREG. At the end of the incubation oocytes were freed of surrounding cumulus cells and luciferase activity was measured in extracts from oocytes. The data are mean ± SEM of three to four independent experiments. *p<0.05 vs MII, **p<0.01 vs MII+AREG. B. Cumulus enclosed oocytes were injected with TPX2 3’UTR Renilla luciferase reporter (RLuc) and polyadenylated firefly reporter (FLuc) and cultured overnight as described above. Quantitative RT-PCR for RLuc and FLuc was performed from oocyte extracts. Exposure to Milrinone or AREG did not change the stability of the reporter. No significant differences between groups.
