Supplementary Figure 2: The aggregates formed by GFP–Cep63 or GFP–Cep152 do not support de novo procentriole formation. | Nature Cell Biology

Supplementary Figure 2: The aggregates formed by GFP–Cep63 or GFP–Cep152 do not support de novo procentriole formation.

From: The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis

Supplementary Figure 2

(a) Expression of GFP-tagged Deup1, Cep63 or Cep152 in U2OS cells. (b,c) Analyses for procentriole formation. The arrowheads and arrows point to typical mother centrioles and GFP-positive aggregates, respectively. The bright SAS-6 foci, which mark the cartwheels, are used as procentriole markers in the quantification analyses. Only interphase cells containing MCD procentrioles, thus in the S or G2 phase, were scored (n = 30 cells from triplicates). GFP–Deup1-expressing cells served as the positive control for de dovo procentriole biogenesis. (d) Endogenous CEP152 exhibits punctate distributions in the GFP–Cep63 aggregates. Such a pattern is in sharp contrast to the ring-shaped distributions of CEP152 in the deuterosomes (arrow in the GFP–Deup1-positive cell) and MCD cradles (arrowheads) (also see Fig. 1h), suggesting that the GFP–Cep63 aggregates are disorganised. Apparently, the punctate CEP152 is unable to induce the cartwheel assembly, not to mention de novo centriole formation. Related to Fig. 1.

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