Supplementary Figure 3: Immunoblotting results for domain mapping.

(a) Mapping of the Deup1- and CEP63-interactiing domains of Cep152. The numbers in the diagrams indicate amino acid positions. Exogenous Cep152, its mutants, or firefly luciferase (Luc) was expressed alone or together with Flag-Deup1 in HEK293T cells, as indicated. Coimmunoprecipitation was then performed using anti-Flag resin. Luciferase served as the negative control. The asterisks indicate the positions of full-length fusion proteins. (b) Mapping of the CEP152-interacting domain of Deup1. Flag-tagged Deup1 or mutants were expressed in HEK293T cells. Coimmunoprecipitation was then performed. (c) Mapping of the CEP152-interacting domain of Cep63. Flag-tagged Cep63 or mutants were expressed in HEK293T cells. Coimmunoprecipitation was then performed. These results are summarised in Fig. 1j.