Supplementary Figure 4: Effect of Deup1 or Cep63 RNAi in MTECs at ALI d 3. | Nature Cell Biology

Supplementary Figure 4: Effect of Deup1 or Cep63 RNAi in MTECs at ALI d 3.

From: The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis

Supplementary Figure 4

(a) Cep152 failed to exhibit the ring-shaped deuterosome localization upon Deup1 RNAi. The arrows indicate MCD procentriole. The insets are magnified 2× to show typical de novo procentrioles (arrowheads). (b-d) Ablation of Cep63 alone in MTECs does not block deuterosome formation and centriole amplification. MTECs were infected with lentivirus to express control (Ctrl-i) or Cep63-specific (63-i1 or 63-i2) shRNA together with GFP–Cetn1. The GFP-positive cells at ALI d 3 were sorted out using FACS and subjected to immunoblotting to show RNAi efficiency. α-Tubulin served as the loading control. The insets are magnified to show MCD procentrioles. The statistical results for centriole numbers are presented in Fig. 6d.

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