Supplementary Figure 3: qPCR data for the expression of seven PrE differentiation stage markers used in Fig. 2b,c.

Each dot represents the gene expression pattern of single cells derived from E3.25 ICM (purple), E3.5 PrE (light green), and E4.5 PrE (dark green) cells with Y-axis indicating the estimated copy number (86 cells in total including 33 cells from 4 embryos for E3.25, 22 cells from 3 embryos for E3.5 PrE, and 31 cells from 3 embryos for E4.5 PrE). The within-group means and the binning thresholds are shown as horizontal dotted lines (light grey) and horizontal solid lines (dark grey), respectively.