Supplementary Figure 1: Performance of spike RNA amplification. | Nature Cell Biology

Supplementary Figure 1: Performance of spike RNA amplification.

From: Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages

Supplementary Figure 1

Each blue line represents the outcome of spike RNA amplification for each experimental sample that is used for microarray (66 cells in total including 36 cells from 6 embryos for E3.25, 22 cells from 3 embryos for E3.5, and 8 cells from one embryo for E4.5). Box plot shows the performance of spike RNA amplification for all samples including those used only for additional qPCR (grey, 154 cells in total including 50 cells from 6 embryos for E3.25, 43 cells from 3 embryos for E3.5 and 61 cells from 3 embryos for E4.5). Those single-cell cDNAs of highest quality with minimal deviation from the ideal value (red line) are processed for microarray analysis. Based on this performance, we defined 20 copies as the minimum amount of mRNAs that we can detect quantitatively.

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