Supplementary Figure 5: The pBpa-labelled Der1RN-Myc transmembrane mutant is properly assembled with the HRD-ligase but displays alterations in the crosslinking to its interaction partners.
From: Der1 promotes movement of misfolded proteins through the endoplasmic reticulum membrane
a, Δder1 Ubc7C/S CPY*-HA cells were transformed with high-copy plasmids encoding either pBpa-modified Der1-Myc (derived from pMM075), Der1RN-Myc (derived from pMM074) or unlabelled Der1-Myc (pMM075). Digitonin-solubilised membranes of the total extract were subjected to immunoprecipitation with anti-Myc antibodies (left and right panel). b, As in a but the microsomes were solubilised with NP40 before precipitation of the Der1-Myc constructs.
