Supplementary Figure 3: Subcellular localization of endogenous MLKL and RIP3 in HT29 cells.
From: Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis
Control shRNA (left panel) or MLKL shRNA (right panel) HT29 cells were treated with TSZ as indicated. Subcellular localization of (a) MLKL or (b) RIP3 was detected by confocal imaging of anti-MLKL antibody or anti-RIP3 antibody staining, respectively. Cell morphology was shown by F-actin probe phalloidin staining. DAPI was used for nuclear staining. White arrows indicate membrane localization. Scale bar, 10 μm.
