Supplementary Figure 6: Extracellular calcium is involved in necroptosis in JurkatFADD−/− and J774A.1 cells.
From: Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis
JurkatFADD−/− or J774A.1 cells were cultured with/out calcium and treated with DMSO control or TSZ as indicated. (a), (c) Cell survival was determined by PI staining. Results shown are averages ± s.e.m. from three independent experiments. *P<0.05, **P<0.01. Statistics source data for Supplementary Fig. 6 can be found in Supplementary Table 2. (b), (d) JurkatFADD−/− or J774A.1 cells expressing control shRNA, MLKL shRNA or cells cultured in calcium free medium were treated with TSZ as indicated. Cells were harvested and the Fluo4 fluorescent cells were determined by FACS analysis using Fluo4. (e), (f) Immunoblot analysis of JuarkatFADD−/− (e) or J774A.1 (f) clones expressing control-shRNA or MLKL-shRNA with anti-MLKL or anti-Actin antibodies. Uncropped images of western blots are shown in Supplementary Fig. 8.
