Supplementary Figure 3: Mitochondrial dysfunction in Drosophila pink1 mutants is complemented by dNK.

(a) Expression of dNK partially reverses metabolic shifts in pink1 mutants. Orange and cyan correspond to metabolites that are respectively upregulated and downregulated to a significant level. The statistical significance was determined using Welch’s Two Sample t-test (n = 8). See also Supplementary Table 6. (b) dNK expression increases mtDNA levels in pink1 mutants. The ratio of mtDNA to nDNA was measured by real-time PCR in flies with the indicated genotypes (mean±SD, n = 6 per genotype, asterisks, one-way ANOVA with Bonferroni’s multiple comparison test). (c) Expression of dNK restores the levels of mitochondrial respiratory complexes in pink1 mutants. Whole-fly lysates were analysed by western blot analysis using the indicated antibodies. (d) dNK expression rescues ATP levels in pink1 mutants. Data are shown as the mean±SD (n = 6 per genotype, asterisks, one-way ANOVA with Bonferroni’s multiple comparison test). (e) dNK expression enhances respiration in pink1 mutants. Data are shown as the mean±SD (n = 3 per genotype, asterisks, two-tailed unpaired t test). See Supplementary Table 9 for statistics source data. (f) dNK expression suppresses motor impairment in pink1 mutants. Flies were tested using a standard climbing assay (mean±SEM, n = 100 flies per genotype, asterisks, one-way ANOVA with Dunnett’s multiple comparison test). (g) dNK expression suppresses flight muscle defects observed in pink1 mutants. dNK expression rescues mitochondrial defects in pink1 mutants (my, myofibrils; m, mitochondria; yellow outlines, mitochondria). See also Supplementary Figs 3 and 9.