Supplementary Figure 7: Dietary supplementation with deoxyribonucleosides or folic acid suppresses pink1 mutant phenotypes.

(a) Suppression of flight muscle degeneration and mitochondrial defects in pink1 mutants by dNs or FA. Ultrastructural analysis of the indirect flight muscles from pink1 mutant flies raised on dNs or FA-supplemented food (my, myofibrils; m, mitochondria; yellow outlines, mitochondria). (b) Dietary supplementation with dNs or FA rescues the thoracic defects of pink1 mutants. pink1 mutants were exposed to a dNs or FA-supplemented diet after egg laying. P-values are indicated (chi-square two-tailed, 95% confidence intervals, n = 454 for normal diet, n = 448 for +dNs and n = 669 for +FA). (c) Dietary supplementation with dNs or FA rescues the flight defects of pink1 mutants. Data are shown as mean±SEM (n = 150 flies per condition). Statistically significant values relative to pink1B9 are indicated by asterisks (one-way ANOVA with Bonferroni’s multiple comparison test). Datasets labelled ‘control’ and ‘pink1B9’ are also used in Supplementary Fig. 4c. (d) and (e) Dietary supplementation with dNs or FA during adult stage rescues the motor impairment of pink1 mutants. pink1 mutants were exposed to a dNs or FA supplemented diet after eclosion. Flies were tested using a standard climbing assay (mean±SEM, n = 100 flies per condition). Statistical significance is indicated (two-tailed unpaired t test). See also Supplementary Fig. 7.