Supplementary Figure 1: Loss of Pink1 results in a metabolic stress response.

RNA and metabolites were isolated from 3-day-old control and pink1 male flies. (a) Quantitative RT–PCR validation for a subset of genes found to be upregulated in the microarray analysis of Drosophila pink1 mutants and that encode for metabolic enzymes. Circled numbers are related to the metabolic reactions shown in b. Data are shown as the mean±SEM, n values are indicated in the bars, asterisks, two-tailed unpaired t test. See also Supplementary Fig. 1 and Supplementary Table 1–3 and 9 for statistics source data. (b) Coordinated changes in metabolite abundance upon loss of pink1 function. On the left, the relative levels of selected metabolites in pink1 mutant flies, detected through GC-MS and LC-MS/MS analysis. A schematic diagram is shown in which these metabolites are depicted with respect to the selected upregulated transcripts. Orange and cyan correspond to metabolites that are respectively upregulated and downregulated to a significant level. The statistical significance was determined using Welch’s Two Sample t-test (n = 8). See also Supplementary Fig. 1 and Supplementary Table 4 and 5.