Supplementary Figure 2: Disrupting the TRN-epidermis attachment is not sufficient to cause buckling and attachments are intact in unc-70 mutants.
From: Mechanical control of the sense of touch by β-spectrin
a, AVM shape during dorsal (top) and ventral (bottom) bending in him-4(e1267) mutants lacking full hypodermal attachment. b, Neuron curvature vs. body curvature in him-4(e1267) mutants. N = number of animals and n = number of still images; collected during n = 4 imaging sessions. c, UNC-70 is not required for TRN attachment. (c) Schematic showing the position of the ALML and AVM. The slab visualizes the arrangement of muscles (blue) and TRNs (green) in respect to the body axis. Individual attachment sites linking body wall muscles and TRNs to the hypodermis are shown in magenta. d, f, h, Representative micrographs anti-myotactin showing attachment sites in (d) control, (f) unc-70(e524) and (h) unc-70(n493) mutant animals. e, g, i, Distribution of interpunctum intervals for attachment sites stained in (e) control, (g) unc-70(e524) and (i) unc-70(n493). Distributions were fitted to a Gamma-distribution with the median location and the residuals from each fit are shown above each graph. N = number of interpunctum intervals analysed for each genotype; more than 25 images analysed for each genotype during n = 3 imaging sessions.
