Supplementary Figure 6: Force-RhoA activity relationship.
From: Interplay of RhoA and mechanical forces in collective cell migration driven by leader cells
Force was plotted against Rho for cells identically positioned in a finger in normalized coordinates. This Figure is the equivalent of Fig. 6B for a normalized ”average finger” derived from several independent experiments. Points have been binned according to their position along and across the fingers and average force and Rho activity values within these bins are reported on this graph. Blue points: longitudinal direction, Red points: transverse direction. The line is the sensitivity as measured in the confocal plane (Fig. 6B). It describes well most of the points with the exception of the leaders (asterisk). Error bars are SEMs. Longitudinal direction: n = 30 cells assessed from 7 experiments. Transverse direction: n = 20 cells assessed from 5 experiments.
