Supplementary Figure 4: FRAP measurements on E-cadherins and actin.
From: Actin dynamics modulate mechanosensitive immobilization of E-cadherin at adherens junctions
(a) Typical E-cad FRAP signal for a doublet treated with Y27632 at 5 μM, in control conditions and treated with nocodazole at 10 μM. (b) E-cad recovery time for doublets treated with latrunculin at 1 μM (n = 15 doublets), with Y27632 at 5 μM (n = 21 doublets), in control conditions (n = 24 doublets), with nocodazole at 10 μM (n = 15 doublets) and with jasplakinolide at 100 nM (n = 12 doublets). The p-values are calculated from unpaired Students t-tests. (c) Typical actin FRAP signal for a doublet treated with Y27632 at 5 μM, in control conditions and treated with nocodazole at 10 μM. (d) E-cad recovery time in the dim region and the bright region of the same doublet (n = 22 doublets). The p-value is calculated from a paired Students t-test.
