Supplementary Figure 5: Features of the E-cadherin distribution at the junction and cortical tension of single cells.

Data in graphs a–c correspond to the same drugs and concentrations: Y27632 at 2 μM (n = 49 doublets), 5 μM (n = 24 doublets), 10 μM (n = 44 doublets) and 20 μM (n = 23 doublets); blebbistatin at 1 μM (n = 22 doublets), 5 μM (n = 17 doublets) and 50 μM (n = 12 doublets); nocodazole at 10 μM (n = 40 doublets); latrunculin at 1 μM (n = 11 doublets), and 5 μM (n = 22 doublets) and jasplakinolide at 100 nM (n = 18 doublets) (*, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 10 − 4). P-values are calculated from paired Students t-test. The whole distributions of the data values are also shown. a Average full-width at half maximum (FWHM) of the E-cad puncta on the dim side and on the bright side in control conditions (grey) and upon drug treatment. The error bars represent the standard deviation. The whole distribution is also shown. b Inter-cluster distance, contact radius and mean E-cadherin signal along the ring in control conditions (grey) and upon drug treatment. The same statistical tests as a. were applied. c E-cadherin density on the ring, at the center of the contact and on the membrane (as depicted in the schematic) in control conditions (grey) and upon drug treatment. d Image of a single cell deformed by negative pressure (left). Cortical tension of single cells for different drug treatment: latrunculin 1 μM (n = 8 doublets), Y27632 5 μM (n = 8 doublets), control (n = 12 doublets), nocodazole 10 μM (n = 9 doublets) and jasplakinolide at 100 nM (n = 10 doublets) (right).