Supplementary Figure 1: Digital procedure for rotation compensation and comparison of E-cadherin junctional distribution for different cell types. | Nature Cell Biology

Supplementary Figure 1: Digital procedure for rotation compensation and comparison of E-cadherin junctional distribution for different cell types.

From: Actin dynamics modulate mechanosensitive immobilization of E-cadherin at adherens junctions

Supplementary Figure 1

(a) Top view (TV1-4) and side view (SV1-4) of a 3D stack used for determining the coordinates of the contact plane. TV1-SV1: deconvoluted stack as imaged in confocal microscopy. TV2-SV2: deconvoluted stack after rotation along the z-axis. TV3-SV3: deconvoluted stack in the referential of the contact. TV4-SV4: raw data in the referential of the contact (scalebar 5 μm). (b) Kymograph of a portion of the E-cad ring before and after image registration (scale bar 5 μm). (c) Typical ring of a MDCK-E-cadGFP doublet (n = 24 cell doublets, 4 days) (left) and an embryonic stem cell E-cadGFP doublet (n = 26 cells, 3 days) (right) (scalebar 5 μm). (d) Western blot of alpha catenin for control S180 cells and cells after alpha catenin knockdown. Cells used to look at the doublets had all the known phenotype of alpha catenin knockdown junctions displaying transient and dynamic cadherin clusters.

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