Supplementary Figure 2: Measurements of doublet deformation.
From: Actin dynamics modulate mechanosensitive immobilization of E-cadherin at adherens junctions
(a) Five rings and their anisotropy vector (top line). Same rings after rescaling and alignment of the anisotropy (bottom line) (scalebar 5 μm). (b) Superimposition of n = 5, 20 and 110 rings after image processing taken. (c) Side view schematic of the doublet deformation. (d) Images of the 2 slices taken 1.5 μm above and below the contact used to determine the contour of the cell (top and bottom). Image of the junction with anisotropy and deformation vectors (middle) (scalebar 5 μm). (e) Extreme cases where d1 and d2 have opposite directions (isotrope ring) and same direction (anisotrope ring) (scalebar 5 μm). (f) Anisotropy orientation as a function of the deformation orientation taken during contact formation and at steady state (n = 28 doublets with a total number of 572 time-points).
