Supplementary Figure 1: Characterization of liver and lung metastatic potential of SW620 parental and LiM2 cells.
(a) Invasion capacity of SW620 parental and LiM2 cells was measured using matrigel-coated Boyden chambers. Results represent values of three independent experiments where each cell line was seeded in six Boyden chambers and five fields per chamber were analysed and average for each chamber obtained (n = 18 chambers per group). Statistical significance was calculated using two-tailed Mann Whitney test; the box extends from 25 to 75 percentile where black line within the box represents median, whiskers extend from 10 to 90 percentile. (b) In vivo proliferation of parental and LiM2 cells (0.5 × 105) injected into cecum of nude mice. Bioluminescent images were taken at day 54. n = 8 (Parental) and n = 9 (LiM2) number of mice used for each cell line. Statistical analysis was done using two-tailed Mann Whitney test and no statistically significant differences were found. (c) Percentage of lymph node and liver metastasis as well as lung metastasis in mice that developed liver metastasis on intracecum injections of parental and LiM2 cells determined post-mortem. Number of mice (n) used is defined in (b). Statistical significance was calculated using one-sided Fisher’s exact test. (d) Representative bioluminescent images of intestine, liver and lung from mice injected intracecum with SW620 parental and LiM2 cells. Data is presented as average plus s.d.
