Supplementary Figure 2: Kif7L130P mutant cells have long cilia and abnormal axonemal structure. | Nature Cell Biology

Supplementary Figure 2: Kif7L130P mutant cells have long cilia and abnormal axonemal structure.

From: The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment

Supplementary Figure 2

(a) The diameter of proximal and distal region was measured from neural tube cilia from e10.5 wild-type and Kif7L130P embryos. The width of proximal cilia was 0.21 ± 0.02 μm in wild-type and 0.20 ± 0.02 μm in Kif7L130P. The width of distal cilia was 0.12 ± 0.02 μm in wild-type and 0.08 ± 0.01 μm in Kif7L130P (n = 50 cilia were measured for each genotype; P < 0.001 by one-way ANOVA analysis). Error bars represent the s.d. (b) Low power s.e.m. of the node of an e8.0 wild-type embryo (black square). (c) Node cilia from e8.0 wild-type and Kif7L130P mutant embryos. Scale bar, 2 μm. (d) TEM images of transverse sections of Kif7L130P neural tubule cilia (4 examples). Arrows indicate singlet microtubules. Scale bars, 50 nm.

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