Supplementary Figure 4: Kif7 associates with microtubule in vivo. | Nature Cell Biology

Supplementary Figure 4: Kif7 associates with microtubule in vivo.

From: The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment

Supplementary Figure 4

(a) Protein domain analysis of mouse Kif7 and KIF4. Motor domain is in green, neck in pink and coiled-coil in purple. Note the long linker between the neck and the first coiled-coil of Kif7, which may inhibit its ability to act as a processive motor. Arrows and numbers indicate the positions of respective domains in amino acid. (b) Co-immunoprecipitation of Flag- and GFP-tagged full-length Kif7 in 293T cells. (c) Expression of C-terminal GFP-tagged Kif7-full length and Kif7560 constructs in HEK293T cells visualized by confocal fluorescent microscope. DAPI in blue. Scale bar, 8 μm. (d) Deletion analysis of Kif7 for in vivo microtubule bundling activity and dimerization.

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