Supplementary Figure 5: Purified Kif7₅₆₀-GFP directly binds to microtubules.

(a) Purified Kif7₅₆₀-GFP for in vitro analysis. Asterisk indicates bacterial protein Arna (Bifunctional polymyxin resistance protein Arna). (b) 70 nM Kif7₅₆₀-GFP binds to X-rhodamine-labelled microtubules in the presence of 1 mM MgATP or 2 mM AMP-PNP. Scale bar, 5 μm.