Supplementary Figure 7: Kif7L130P mutant cilia have defects in IFT dynamics. | Nature Cell Biology

Supplementary Figure 7: Kif7L130P mutant cilia have defects in IFT dynamics.

From: The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment

Supplementary Figure 7

(a–b) Representative kymographs generated from time lapse imaging of wild-type and (C–D) Kif7L130P primary cilium (Supplementary Videos 1 and 2). (a) and (c) are taken from Fig. 3. The base and tip of the cilium are indicated with arrowheads. Asterisks indicate stationary IFT88 puncta associated with the initiation of retrograde trafficking. Horizontal scale bar (distance), 1 μm. Vertical scale bar (time), 7.5 s. Enlarged portion of kymographs are highlighted (yellow rectangles). Asterisks show the position of stationary IFT88 puncta. Our kymograph analysis shows IFT88–GFP (4/200 retrograde trains) switches tracks in the wild-type cilia very rarely, whereas significantly more trains (79/214 retrograde trains) switch tracks in Kif7 mutants. (e) Numbers of stationary IFT88–GFP puncta per cilium in wild-type and Kif7L130P (n = 50 cilia were imaged for each condition and were pooled from 5 independent experiments; P < 0.0001 by student t-test). The error bars represent s.d.

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