Supplementary Figure 1: Adult neural stem cells express functional N-cadherin.

(a) Immunofluorescent detection of GFAP (red), β-catenin (blue) and γ-tubulin (green) in the wall of the lateral ventricle of wild type mice. (b) Wholemount staining for N-cadherin (red) and GFP (green) in GFAP–eGFP mice. Note that the GFP⁺ cell shown is also positive for N-cadherin (white arrowheads). Stainings at specific confocal levels are shown (z). Lower panel; higher magnification of z-stack projection showing a GFP⁺, B cell. (c) Immunofluorescent detection of the ependymal marker S100β (red), GFP (green) and DAPI (blue) in preparations from co-culture experiments using SEZ homogenates from GFAP–eGFP mice on N-cadherin overexpressing L929 cells. (d) Immunofluorescent detection of GFAP (green), Ki67 (cyan) and N-cadherin (red) in the SEZ of wild type mice. Shown is the staining at a specific confocal level (z). Right set of smaller panels show a higher magnification of the area outlined by the white line in the left panel, at the specified confocal levels. Non-proliferative GFAP⁺ cells show higher levels of N-cadherin staining (full arrowheads) whilst activated to proliferate, Ki67-GFAP double positive cells, have lowered N-cadherin levels (empty arrowheads). Nuclei are counterstained with DAPI (blue), and the dashed white lines mark the ventricle limit. Scale bars: (a,c,d) 5 μm; (b) 10 μm.