Supplementary Figure 2: N- and E-cadherin expression, and pinwheel structure integrity in GFAP(Cre);Cdh2^Δ mice.

(a) Immunostaining for GFAP (red) and N-cadherin (green), and (b) E-cadherin (green) in SEZ coronal sections of adult mice with the conditional deletion of N-cadherin in GFAP⁺ cells (GFAP(Cre);Cdh2^Δ) or control littermates (Cdh2^floxed). DAPI was used for counterstaining (blue). The dashed white lines mark the ventricle limit. Note the absence of signal for N-cadherin in the SEZ of conditionally targeted animals, whilst E-cadherin staining remained apparently unaffected. (c) Immunofluorescent staining for the detection of S100β (red) and GFAP (green) in tissue from Cdh2^floxed and GFAP(Cre);Cdh2^Δ mice, in wholemount preparations and (d) coronal sections. Scale bars: 10 μm.