Supplementary Figure 3: Partial repression of CH-TOG/CKAP5 or low dose Taxol treatment suppresses high chromosome number variability after loss of CHK2 or AURKA overexpression without affecting normal cell cycle progression.

(a) Protein levels of ch-TOG and Aurora-A in HCT116 cell lines stably overexpressing AURKA after transient siRNA-mediated knockdown of CH-TOG/CKAP5. A representative western blot is shown and relative protein levels of ch-TOG were quantified. (b) Protein levels of ch-TOG in stable CH-TOG/CKAP5 knockdown cell lines derived from parental HCT116 or HCT116-CHK2^−/− cells. A representative western blot is shown and relative protein levels were quantified. (c) Karyotype analyses using CEP-FISH of HCT116 and HCT116-CHK2^−/− single cell clones grown in the presence or in the absence of 0.2 nM Taxol for 30 generations. The proportion of cells that deviate from the modal chromosome number of chromosome 7 and chromosome 15 were calculated (100 cells analysed per condition). (d) Partial loss of CH-TOG/CKAP5 does not affect normal cell cycle progression. Single cell clones were subjected to FACS analyses and representative cell cycle profiles are given. (e) Low dose Taxol does not affect normal cell cycle progression in cells after treatment with low dose Taxol. Single cell clones derived from HCT116 cells overexpressing AURKA (clone 1) or derived from HCT116-CHK2^−/− cells were generated in the presence of DMSO or 0.2 nM Taxol. After 30 generations cells were subjected to FACS analyses and representative cell cycle profiles are given. Detailed data on karyotype analyses can be found in the Supplementary Table 1. Statistic source data for Supplementary Fig. 3 can be found in the Supplementary Table 2.