Supplementary Figure 8: Adult Lgr5⁺ cells beyond the hilum are capable of OSE generation.

Confocal z-stack images of near-native Lgr5-KI/Rosa26-tdTomato ovary slices 2 days (2d), 2 weeks (2w) and 4 months (4m) post adult-induction (p.i.). At 2 days p.i., 29.5% of Lgr5–EGFP^hi cells have activated the tdTomato reporter (432 tdTomato⁺ cells out of 1463 Lgr5-GFP^hi OSE cells counted). These cells rapidly expanded to form tdTomato⁺ clones throughout the ovary surface encompassing the ovary hilum and anterior ovary 2w and 4m p.i. Arrowheads point to larger-sized clones at the edges or atop corpora lutea. White dashed line demarcates ovary-mesovarian junction. Yellow dashed lines demarcate follicles. Scale bars: 10 μm.